#Youtube bio rad western blot how to#
#Youtube bio rad western blot serial#

Prestained and unstained molecular weight standards for protein electrophoresis applications including SDS-PAGE, western blotting, 2-D PAGE, and isoelectric focusing (IEF).įind the right Bio-Rad protein gel for your application. Protein Ladders and Standards (Markers).Assembly of the blotting sandwich and electroblotting are shown along with the steps for protein detection using a colorimetric assay. This video demonstrates SDS-PAGE separation of proteins using the Comparative Proteomics Kit II: Western Blot Module. Assay steps, including addition of samples to the microtiter plate strip and washing steps, are shown.

#Youtube bio rad western blot serial#

Serial dilutions of standards are performed and used for quantitation of test samples. This video demonstrates a quantitative ELISA (enzyme-linked immunosorbent assay) using the ELISA Immuno Explorer™ Kit. Assay steps for detecting the presence or absence of an antibody, including addition of samples to the microtiter plate strip and washing steps, are shown. This video demonstrates a qualitative ELISA (enzyme-linked immunosorbent assay) using the ELISA Immuno Explorer™ Kit. This technique is used to test whether antibodies recognize specific antigens. This video demonstrates the Ouchterlony double immunodiffusion assay. Cheek cells and onion epidermal cells are stained and mounted on a slide.

#Youtube bio rad western blot how to#

This video demonstrates how to create a wet mount of cells on a microscope slide.

Bacteria are serially diluted and plated onto Petri plates using aseptic technique. This video demonstrates how to quantitate bacteria using the serial dilution and plate count method.

The method for mounting bacteria on a microscope slide using aseptic technique is shown. This video demonstrates the Gram staining method for identifying unknown bacteria. This method is used for testing the susceptibility of bacteria to different antibiotics. This video demonstrates how to perform a modified Kirby-Bauer, or disk diffusion test. Proper use of a bunsen burner, inoculation loop, and aseptic technique are shown. This video demonstrates the streak plate method for isolating a single colony of bacteria from a culture. Proper pouring of Petri plates, including labeling, is shown. LB agar is made, showing use of an electronic balance and addition of selection components, ampicillin and arabinose, to media. This video demonstrates how to make microbiological media. Techniques include use of a Bunsen burner, transferring bacterial cultures from Petri plate to a liquid culture or from liquid to liquid culture using inoculation loops or needles. This video demonstrates basic microbiological aseptic techniques used for transferring bacteria from one culture to another. Calculations to determine concentration of the unknown are shown using the m1v1 = m2v2 formula. Proper use of graduated cylinders for volume measurement and use of a burette for titration using the pH indicator phenolphthalein are shown. This video demonstrates how to determine the concentration of an unknown base by titration. The example is for making a 1 M NaCl solution and includes weighing the material on an electronic balance and measurement of 100 ml of total solution using a volumetric flask. This video demonstrates the proper method for making a molar solution. The parts of the instrument, calibration with standard pH buffers, and sample measurement are shown. This video demonstrates the proper use of a stationary pH meter. This video demonstrates the proper use of an electronic balance. Different percentages of alcohol are used in this chromatographic separation technique. This video demonstrates the separation of Kool-Aid drink mix pigments based on differences in polarity. The parts of the micropipet are explained along with how to set the volume, select and apply the pipet tip, and transfer liquids. This video demonstrates how to use an adjustable-volume micropipet. This video demonstrates the proper use of three common types of pipet pumps - tri-valve, syringe, and electronic - which are used with serological pipets. This video demonstrates the proper use of a disposable plastic transfer pipet.